2. Stability of the kit is as per the expiry date mentioned

on the label.

Additional Material Required

Sterile plating loops (10 µL), biosafety hood with Bunsen

burner, activated 2% glutaraldehyde solution, distilled

water, microscope with oil immersion lens, cedar wood oil.

Specimen Collection and Preparation

Collect specimen prior to use of antimicrobial agent.

Wherever possible, indicate clearly that patient is on

antitubercular drugs.

CSF

Collect as much as possible in a syringe, clean skin with

alcohol before aspirating specimen.

Body Fluids

Disinfect the site and collect specimen with aseptic

precautions.

Sputum

Collect 5 to 10 mL in a sterile container from an early

morning specimen of deep productive cough. For induced

specimen use sterile saline. Have patients rinse mouth

with water to minimize specimen contamination with

food particles, mouthwash, or oral drugs.

Urine

As organisms accumulate in the bladder overnight, first

morning void provides best yield. Collect midstream clean

catch urine, first morning catheterization/suprapubic taps

in sterile containers.