Infection with Mycobacterium tuberculosis remains a major
public health problem. The epidemic of tuberculosis and
multi drug resistant tuberculosis reflects the failure of public
health and social programs towards prompt treatment
of infected cases and screening of high-risk population.
While culture, isolation and sensitivity of Mycobacterium
tuberculosis from patient groups using standard methods
remain the gold standard for Mycobacterium tuberculosis
detection and effective and swift treatment worldwide, acid
fast bacilli staining is the first line microscopic procedure
NOVACHROM is a reagent for laboratory use only.
NOVACHROM Rapid Two Step Cold AFB Stain comprises
a. AFB stain (A) — carbol fuchsin
b. AFB stain (B) — counterstain with decolorizer
Rapid Two Step Cold AFB Stain is provided as a ready
to use stain set. It is used for screening of Mycobacterium
tuberculosis from biological specimen such as sputum,
CSF and urine. It is also used in the identification of
Mycobacterium tuberculosis from isolated culture. It is a
modification of Kinyoun’s cold stain.
Carbol fuchsin forms acid insoluble complex with mycolic
and present on the Acid Fast Bacilli and renders red/pinkish
red color to Mycobacterium tuberculosis. Other elements
present in the smear take up counterstain (Methylene blue)
and are stained bluish. Rapid Two Step Cold AFB Stain avoids
the extra decolorization step associated with traditional
staining techniques as the decolorizing component is
incorporated within the counterstain. The staining system
is simple to perform and very much reproducible. It has a
sensitivity comparable to the traditional Ziehl-Neelsen hot
staining method and also as compared to Acid Fast Bacilli
culture results. It is a clinically proven, easy to use, time,
labor and cost saving. This simplicity of staining makes this
an ideal screening tool for Mycobacterium tuberculosis.
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